Abstract
BackgroundmiRNA-154 (miR-154) has been identified as a tumor suppressor in several types of human cancers. However, its clinical significance in colorectal cancer (CRC) is still unclear. The aim of this study was to analyze the association of miR-154 expression with clinicopathologic features and prognosis in CRC patients.MethodsQuantitative RT-PCR was performed to evaluate miR-154 levels in 169 pairs of CRC specimens and adjacent noncancerous tissues. Then, the associations of miR-154 expression with clinicopathological factors or survival of patients suffering CRC were determined.ResultsThe expression levels of miR-154 in CRC tissues were significantly lower than those in corresponding noncancerous tissues (P < 0.001). Decreased miR-154 expression was significantly associated with large tumor size, positive lymph node metastasis, and advanced clinical stage. Moreover, the univariate analysis demonstrated that CRC patients with low miR-154 expression had poorer overall survival (P = 0.006). The multivariate analysis identified low miR-154 expression as an independent predictor of poor survival.ConclusionsThese findings suggested that miR-154 downregulation may be associated with tumor progression of CRC, and that this miR may be an independent prognostic marker for CRC patients.
Highlights
MiRNA-154 has been identified as a tumor suppressor in several types of human cancers
The multivariate analysis identified low miR-154 expression as an independent predictor of poor survival. These findings suggested that miR-154 downregulation may be associated with tumor progression of colorectal cancer (CRC), and that this miR may be an independent prognostic marker for CRC patients
Downregulation of miR-154 confers poor prognosis in CRC patients Using the Kaplan-Meier method and log-rank test, we found that the overall survival of CRC patients with low miR-154 expression was significantly shorter than those with high miR-154 expression (P = 0.006; Fig. 2)
Summary
MiRNA-154 (miR-154) has been identified as a tumor suppressor in several types of human cancers. Its clinical significance in colorectal cancer (CRC) is still unclear. The aim of this study was to analyze the. C association of miR-154 expression with clinicopathologic features and prognosis in CRC patients. I Methods: Quantitative RT-PCR was performed to evaluate miR-154 levels in 169 pairs of CRC specimens and adjacent noncancerous tissues. The associations of miR-154 expression with clinicopathological factors or. T survival of patients suffering CRC were determined
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