Abstract
BackgroundMicroRNA-452 (miR-452) was previously reported to be dysregulated in several types of human cancers and involved in tumor progression. The aim of this study was to investigate the clinical significance and prognostic value of miR-452 expression in human osteosarcoma.MethodsThe expression of miR-452 was detected in 95 pairs of osteosarcoma specimens and adjacent noncancerous bone tissues using quantitative RT-PCR (qRT-PCR) assay. Then, the association of miR-452 levels with clinicopathological features and prognosis was analyzed. The roles of miR-452 in regulating osteosarcoma cell proliferation, apoptosis, and invasion were evaluated in vitro.ResultsmiR-452 expression was significantly downregulated in osteosarcoma tissues than those in corresponding noncancerous bone tissues (P < 0.001). Decreased miR-452 expression was linked to larger tumor size, high tumor grade, advanced clinical stage, distant metastasis, and shorter overall survival. Multivariate Cox regression analysis confirmed that low level of miR-452 expression predicted poor prognosis independently. miR-452 overexpression in MG-63 cells suppressed cell proliferation, promoted cell apoptosis, inhibited cell invasion, and led to decreased BMI1 protein levels.ConclusionsThese findings suggest that miR-452 downregulation may be involved in osteosarcoma formation and progression and that miR-452 would serve as a novel prognostic biomarker for patients with this disease.
Highlights
A Results: miR-452 expression was significantly downregulated in osteosarcoma tissues than those in corresponding noncancerous bone tissues (P < 0.001)
E Conclusions: These findings suggest that miR-452 downregulation may be involved in osteosarcoma formation and progression and that miR-452 would serve as a novel prognostic biomarker for patients with this disease
Prognostic value of miR-452 in osteosarcoma we evaluated the prognostic significance of miR-452 expression in patients with osteosarcoma
Summary
Low miR-217 and miR-646 expression levels were unfavorable prognostic factors for overall survival of osteosarcoma patients [13]. Ectopic expression of miR-30a promoted chemotherapy-induced apoptosis and enhanced chemosensitivity in osteosarcoma [17]. Exploring the roles of miRs in osteosarcoma development and its underlying mechanisms may be helpful for its early diagnosis, accurate assessment, targeted therapy, and prognosis evaluation. Aberrant miR-452 expression has been demonstrated in glioma [21], non-small cell lung. E Paired fresh osteosarcoma specimens and noncancerous bone tissues were collected from 95 patients at The First Affiliated Hospital of Zhengzhou University Complementary DNA (cDNA) was synthesized from isolated RNA using TaqMan MicroRNA Reverse Transcription Kit and miRNA specific stem-loop primers (both from Applied Biosystems, Foster City, CA, USA). The clinical stage was classified according to Enneking’s brief, MG-63 cells were seeded in a 96-well plate, and
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
