Abstract
The effects of human immunodeficiency virus (HIV) on the immune response in patients with cutaneous leishmaniasis have not yet been fully delineated. This study quantified and evaluated the function of memory T-cell subsets in response to soluble Leishmania antigens (SLA) from patients coinfected with HIV and Leishmania with tegumentary leishmaniasis (TL). Eight TL/HIV coinfected subjects and 10 HIV seronegative subjects with TL were evaluated. The proliferative response of CD4+and CD8+T-cells and naïve, central memory (CM) and effector memory (EM) CD4+T-cells in response to SLA were quantified using flow cytometry. The median cell division indices for CD4+and CD8+T-cells of coinfected patients in response to SLA were significantly lower than those in patients with Leishmania monoinfection (p < 0.05). The proportions of CM and EM CD4+T-cells in response to SLA were similar between the coinfected patients and patients with Leishmania monoinfection. However, the median CM and EM CD4+T-cell counts from coinfected patients were significantly lower (p < 0.05). The reduction in the lymphoproliferative response to Leishmania antigens coincides with the decrease in the absolute numbers of both EM and CM CD4+T-cells in response to Leishmania antigens in patients coinfected with HIV/Leishmania.
Highlights
Eight patients with tegumentary leishmaniasis (TL) and human immunodeficiency virus (HIV) infection and 10 HIV seronegative subjects with TL were included in the study
This study demonstrates that a reduction in the lymphoproliferative response to Leishmania antigens coincides with a decrease in the absolute numbers of both effector memory (EM) and central memory (CM) CD4+ T-cells in response to Leishmania antigens in patients coinfected with HIV/Leishmania
The proportion of memory CD4+ T-cells subsets was similar between monoinfected Leishmania and coinfected HIV/Leishmania patients, the median absolute count of EM lymphocytes in coinfected patients was four-fold lower compared with that of Leishmania monoinfected patients
Summary
Eight patients with TL and HIV infection and 10 HIV seronegative subjects with TL were included in the study. We have quantified and assessed the function of memory T-cell subsets in response to soluble Leishmania antigens (SLA) from patients coinfected with HIV and Leishmania with tegumentary leishmaniasis (TL). The CD4+ and CD8+ T-cell subsets from both HIV/ Leishmania coinfected and Leishmania monoinfected patients all proliferated in response to PHA.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
