Abstract
BackgroundBRAF-activated long non-coding RNA (BANCR) has been associated with various types of cancer. Nevertheless, the role of BANCR in clear cell renal cell carcinoma (ccRCC) is still not fully understood. This study aims to investigate the relationship between ccRCC and BANCR.MethodsExpression of BANCR in TCGA renal cancer data sets was analyzed. The expression pattern of BANCR in Immortalized normal human proximal tubule epithelial cell line HK-2 and ccRCC cell lines (ACHN, CAKI-1, A498 and 786-O) was detected by real-time quantitative RT-PCR (qRT-PCR). ccRCC tissues with adjacent normal renal tissues diagnosed by pathological methods from 62 patients were used to detect the expression of BANCR, and its correlation with prognosis of ccRCC patients was assessed by Kaplan-Meier method. The LV-BANCR vector was used to examine the influence of BANCR on the proliferation, migration, invasion, apoptosis and cell cycle distribution of ccRCC cells in vitro.ResultsBANCR was downregulated in renal cancer according to TCGA data sets. Compared with adjacent normal renal tissues and normal human proximal tubule epithelial cell line HK-2, BANCR expression was significantly decreased in ccRCC tissues and ccRCC cell lines, and its low expression was associated with poor prognosis. Moreover, in the condition of BANCR overexpression by LV-BANCR vector, the proliferation, migration, invasion capacity of ccRCC cells was inhibited, while the apoptosis was increased and the G1 cell cycle arrest was induced in vitro.ConclusionsBANCR is downregulated in ccRCC tissues and cell lines, and is associated with ccRCC progression. Thus, BANCR may represent a novel prognostic biomarker and a potential therapeutic target for ccRCC patients.
Highlights
BRAF-activated long non-coding RNA (BANCR) has been associated with various types of cancer
BANCR is down-regulated in clear cell renal cell carcinoma (ccRCC) tissues and cell lines We analyzed the expression of BANCR in TCGA renal cancer data sets
The results indicated that BANCR expression was markedly down-regulated in renal cancer tissues compared to normal renal tissues (p < 0.01) (Fig. 1a)
Summary
BRAF-activated long non-coding RNA (BANCR) has been associated with various types of cancer. The role of BANCR in clear cell renal cell carcinoma (ccRCC) is still not fully understood. Renal cell carcinoma (RCC) is one of the most commonly diagnosed urological cancers in the world [1]. Among all subtypes of RCC, clear cell renal cell carcinoma (ccRCC) accounts. Multitudinous long noncoding RNAs (lncRNA) have shown to be involved in various types of tumorigenesis [9,10,11]. During the last decade, accumulated evidence has demonstrated that lncRNAs have an important role in tumorigenesis, invasion and metastasis of human cancers [12,13,14]. BRAF- activated non-coding RNA (BANCR) has been originally identified in melanoma cells [15]. Wang et al have reported that BANCR promotes endometrial cancer cell invasion and proliferation by modulating ERK/MAPK signaling pathway [19]
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