Abstract
In the spring of 2010, it was discovered that a large proportion of broilers in Sweden were colonized with Escherichia coli producing extended-spectrum beta-lactamases (ESBL) or plasmid mediated AmpC (pAmpC). It was hypothesized that the high prevalence was due to transfer from an upper level in the production pyramid and sampling upwards in the production pyramid was initiated. From 2010 to 2019, all shipments (n = 122) of broiler breeders were screened on arrival to Sweden for the occurrence of ESBL- or pAmpC-producing E. coli using selective methods. Samples of paper linings from shipments of breeders were cultured on MacConkey agar supplemented with cefotaxime (1 mg/L) after pre-enrichment in either MacConkey broth with cefotaxime (1 mg/L), or from late June 2015 in buffered peptone water without antibiotics. ESBL- or pAmpC-producing E. coli was isolated from 43 (35%) of these. Over the years, the proportion of positive imports have decreased and during 2018 and 2019 all imports were negative. In conclusion, the occurrence of ESBL- or pAmpC-producing E. coli in broiler breeders on arrival to Sweden has decreased. Such bacteria have not been detected in any shipments since 2017.
Highlights
In the spring of 2010, it was discovered that a large proportion of broilers in Sweden were colonized with Escherichia coli producing extended-spectrum beta-lactamases (ESBL) or plasmid mediated AmpC
Due to the risk of spread from animals to humans via food, the occurrence of Escherichia coli with resistance to extended spectrum cephalosporins in caecal samples from broilers has since 2010 been investigated with selective culture methods within the Swedish veterinary antimicrobial resistance monitoring program (Svarm) [3]
*Correspondence: stefan.borjesson@liu.se 1 Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), 751 89 Uppsala, Sweden Full list of author information is available at the end of the article it was discovered within the framework of Svarm, that a large proportion of broilers in Sweden was colonized with ESBL- or plasmid mediated AmpC (pAmpC)-producing E. coli (Fig. 1)
Summary
In the spring of 2010, it was discovered that a large proportion of broilers in Sweden were colonized with Escherichia coli producing extended-spectrum beta-lactamases (ESBL) or plasmid mediated AmpC (pAmpC). Due to the risk of spread from animals to humans via food, the occurrence of Escherichia coli with resistance to extended spectrum cephalosporins in caecal samples from broilers has since 2010 been investigated with selective culture methods within the Swedish veterinary antimicrobial resistance monitoring program (Svarm) [3].
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