Decrease of mesophyll conductance to CO2 is a possible mechanism of abscisic acid influence on photosynthesis in seedlings of pea and wheat

Abstract

Treatment of plants by phytohormones is a perspective method of regulation of the plant stress resistance and productivity. However, the mechanisms of phytohormone effects on physiological processes require investigations. The aim of this work was the analysis of the influence of exogenous abscisic acid (ABA) on photosynthesis in seedlings of pea and wheat, and in particular, an estimation of the involvement of the mesophyll conductance to CO2 in the realization of the ABA effects. A standard system for recording of photosynthetic parameters and a system for intracellular measurements of electrical activity were used in the experiments. It was shown that the effect of exogenous ABA on photosynthesis was most prominent 1 day after spraying of a plant. A detailed analysis of photosynthetic processes showed that ABA decreased photosynthetic assimilation of CO2 and increased cyclic electron flow in plants under study; these processes were interconnected. A decrease of the mesophyll conductance to CO2 was probably a mechanism of the decrease in the photosynthetic assimilation of CO2, because ABA did not significantly influence water conductance of a leaf and parameters of the CO2 fixation in Calvin–Benson cycle. It is likely that the decrease of the mesophyll conductance to CO2 was related with a decrease of the plasma membrane conductance to carbon dioxide. Inactivation of H+-ATP-ase and changes in extracellular pH can be a mechanism of this decrease. A decrease of the metabolic component of the electrical resting potential after the ABA treatment testifies in favor of this possibility.