Decrease in glucose transport activity of Friend erythroleukemia cell caused by dimethylsulfoxide, a differentiation-inducing reagent

Abstract

A transport system for d-glucose was found in a Friend erythroleukemia cell line, T-3-Cl-2-O and was characterized as a facilitated diffusion system. d-Glucose transport activity showed a half-saturation concentration of 2.2 mM and was inhibited by mercuric ions, cytochalasin B, phloretin, and stilbestrol, but was not strongly inhibited by phloridzin. Transport of 3-O- methyl- d- glucose was faster than d-glucose and the intracellular concentration of the sugar was found to reach the concentration in the assay medium. The treatment of cells with a differentiation-inducing reagent, dimethylsulfoxide(Me 2SO), for 24 h caused a marked decrease in glucose transport activity due to a decrease in V max. In an induction-insensitive Friend cell line, T-3-K-1, d-glucose transport activity was low in untreated cells and Me 2SO treatment did not cause a significant decrease in transport activity. The results obtained in this study indicate that the decrease in glucose transport activity is not due to the direct effect of Me 2SO on transport activity, but is associated with the induction of differentiation. By immunoblotting cell lysates of T-3-Cl-2-O cells using antibody to human erythrocyte glucose transporter, a single major band having a molecular weight of 52000 was detected, which may be a glucose transporter in Friend cells.