Decrease in alkaline triglyceride lipase in primary cultured hepatocytes from mice with sarcoma 180.

Abstract

Primary cultured hepatocytes from normal mice and mice with Sarcoma 180 were characterized. The viability of freshly isolated hepatocytes from both sources was over 90% and the cells had a relatively stable population of DNA for a minimum of three days. After incubation with (3H)leucine, the syntheses and secretions of (3H)labeled trichloracetic acid-insoluble materials by hepatocytes from both normal and tumor-bearing mice increased similarly. However, the alkaline triglyceride lipase activity of a homogenate of freshly isolated hepatocytes from tumor-bearing mice was one-third that of cells from normal mice. The activity of hepatocytes from tumor-bearing mice increased less during culture than did the activity of cells isolated from normal mice.