Abstract
Decontamination by modified Petroff’s method is being practiced in many laboratories carrying out Mycobacterium tuberculosis culture and drug susceptibility testing. The method exposes mycobacteria to 4% sodium hydroxide for 30min. However, laboratories in developing countries with limited resources might be using a type of centrifuge that does not open during power failures and exposes the mycobacteria to alkali for longer periods. Out of 28 smear-positive specimens processed, 85.7%, 85.7% and 60.7% of specimens showed a positive culture after exposure to alkali for 0.5, 1.0 and 72h. Laboratories compelled to expose the mycobacteria for a longer duration of time can still attempt isolation for culture as only a small amount of bacteria are needed for culture positivity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
