Deconstructing molecular phylogenetic relationship among cultivated and wild Brassica species

Jebi Sudan,Reetika Mahajan,Ravinder Singh,Romesh K Salgotra

doi:10.1007/s10722-021-01198-1

Abstract

Brassica represents an agriculturally important and diverse group of oilseed crops with a long evolutionary history. Molecular markers played an important role in understanding origin and evolution of Brassica species. In the present research, both Single Nucleotide Polymorphisms (SNPs) and Simple Sequence Repeats (SSRs) developed from Brassica juncea L. Czern. were used to find out the phylogenetic relationship between different cultivated and wild Brassica species. A total of 88 SSR and 58 SNP markers were found to be functional across 38 genotypes belonging to ten different taxon groups. The polymorphic markers were able to group the genotypes into three different clusters and showed relatedness among different genomes based on genetic distances. The transferability of these markers serve the purpose of their quick use in cultivar identification, diversity and phylogenetic analysis in those orphan crops species where no or less genomic information is available.

Highlights

Brassicaceae is a highly diverse family that comprises of agriculturally important species including model plant (Arabidopsis thaliana), highly cultivated oilseed crops (Brassica napus, B. rapa, B. carinata and B. juncea), widely utilized vegetable species (B. oleracea), eatable roots (Raphanus sativus) and various herbs (B. nigra and Sinapis alba)
In present research both Single Nucleotide Polymorphisms (SNPs) and Simple Sequence Repeats (SSRs) developed form Brassica juncea were used to find out the phylogenetic relationship between various cultivated and wild Brassica species
The SNPs used in the current study have revealed high cross-genome transferability of more than 59% across the species

Summary

Introduction

Brassicaceae is a highly diverse family that comprises of agriculturally important species including model plant (Arabidopsis thaliana), highly cultivated oilseed crops (Brassica napus, B. rapa, B. carinata and B. juncea), widely utilized vegetable species (B. oleracea), eatable roots (Raphanus sativus) and various herbs (B. nigra and Sinapis alba) This broad array of diversity is mainly because of genetic manipulations caused due to natural evolution of polyploids and amphidiploid hybrids, and traditional breeding including selection followed over the time (Edward et al 2011). No attempts had been made to explore the efficiency of cross species transferability of SNP as well as SSR markers from B. juncea to wild Brassica spp The transferability of these markers serves the purpose of their quick use in cultivar identification, diversity and phylogenetic analysis in those orphan crops species where no or less genomic information is available. We used both SNP and SSR markers developed from Brassica juncea and evaluate them for population, genetic diversity and evolutionary relationship studies in wild Brassica spp

Methods

Results

Conclusion