Abstract
Glucose-sensitive films were prepared through the layer-by-layer (LbL) deposition of hemin-modified poly(ethyleneimine) (H-PEI) solution and DNA solution (containing glucose oxidase (GOx)). H-PEI/DNA + GOx multilayer films were constructed using electrostatic interactions. The (H-PEI/DNA + GOx)5 film was then partially decomposed by hydrogen peroxide (H2O2). The mechanism for the decomposition of the LbL film was considered to involve more reactive oxygen species (ROS) that were formed by the reaction of hemin and H2O2, which then caused nonspecific DNA cleavage. In addition, GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. Therefore, decomposition of the (H-PEI/DNA + GOx)5 film was observed when the thin film was immersed in a glucose solution. (H-PEI/DNA + GOx)5 films exposed to a glucose solution for periods of 24, 48 72, and 96 h indicated that the decomposition of the film increased with the time to 9.97%, 16.3%, 23.1%, and 30.5%, respectively. The rate of LbL film decomposition increased with the glucose concentration. At pH and ionic strengths close to physiological conditions, it was possible to slowly decompose the LbL film at low glucose concentrations of 1–10 mM.
Highlights
Layer-by-layer (LbL) films have previously been prepared by the alternate deposition of polyelectrolytes on a solid surface, assisted by electrostatic interaction [1,2,3].Various other interactions have been recently employed to construct LbL films, such as hydrogen bonding [4,5] and sugar-lectin binding [6,7]
Films consisting of hemin-modified poly(ethyleneimine) (H-PEI) and DNA with glucose oxidase (GOx), and the glucose-induced preparation of thin LbL films consisting of H-PEI and DNA with glucose oxidase (GOx), and the decomposition of these
Results and Discussion was immersed in the H-PEI solution and DNA/GOx mixed solution
Summary
Layer-by-layer (LbL) films have previously been prepared by the alternate deposition of polyelectrolytes (polycation and polyanion) on a solid surface, assisted by electrostatic interaction [1,2,3]. We have recently reported that hydrogen peroxide (H2 O2 ) induced the decomposition of LbL films composed of hemin-modified poly(ethyleneimine) (H-PEI) and DNA [25]. LbL films composed of oxidases have been applied in generated by the reaction of substrates and oxidases. We report the thin LbL films consisting of H-PEI and DNA with glucose oxidase (GOx), and the glucose-induced preparation of thin LbL films consisting of H-PEI and DNA with glucose oxidase (GOx), and the decomposition of these. Glucose-sensitive materials to level of a diabetic patient; LbL films composed of GOx can be applied to an insulin. The blood glucose level of a diabetic patient; LbL films composed of GOx can be applied to. 1. Preparation andand decomposition layer-by-layer films composed hemin, DNA, and glucose oxidase (GOx).
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