Decolourization of the reconstituted dye bath effluent by commercial laccase treatment: Optimization through response surface methodology

Abstract

This paper aims to study the effect of temperature, pH and enzyme concentration on decolourization of separately two reactive textile dyes (Black Novacron R and Blue Bezaktiv S-GLD 150) used in reconstituted dye bath effluent (textile dye and auxiliary components) and in aqueous dye solutions (dye dissolved in deionised water) by a commercial laccase formulation (DeniLite ® IIS). The central composite design (CCD) matrix and response surface methodology (RSM) have been applied to design experiments for the evaluation of the interactive effects of the three most important operating variables: temperature ‘T’ (25–45 ◦C), pH (3.0–7.0), and enzyme concentration ‘EC’ (80–240 U/L) on the enzymatic decolourization of the different synthetic dyes solutions at initial dye concentration of 40 mg/L. The RSM indicated that the optimum parameter values were respectively for the reconstituted Black Novacron R and the Blue