Abstract
A 4-Chloro-2-nitrophenol (4C2NP) decolourizing strain RKJ 700 was isolated from soil collected from a pesticide contaminated site of India and identified as Bacillus subtilis on the basis of the 16S rRNA gene sequence analysis. Bacillus subtilis RKJ 700 decolourized 4C2NP up to concentration of 1.5 mM in the presence of additional carbon source. The degradation pathway of 4C2NP was studied and 4-chloro-2-aminophenol, 4-chloro-2-acetaminophenol and 5-chloro-2-methylbenzoxazole (5C2MBZ) were identified as metabolites by high performance liquid chromatography and gas chromatography-mass spectrometry. Resting cell studies showed that Bacillus subtilis RKJ 700 depleted 4C2NP completely with stoichiometric formation of 5C2MBZ. This is the first report of (i) the degradation of 4C2NP at high concentration (1.5 mM) and, (ii) the formation of 5C2MBZ by a soil bacterium.
Highlights
A 4-Chloro-2-nitrophenol (4C2NP) decolourizing strain RKJ 700 was isolated from soil collected from a pesticide contaminated site of India and identified as Bacillus subtilis on the basis of the 16S rRNA gene sequence analysis
Isolation and Identification of Bacterial Strain A 4C2NP decolourizing bacterial strain RKJ 700 was isolated from soil collected from a pesticide contaminated site of Punjab, India
Strain RKJ 700 was identified as Bacillus subtilis based on the 16S rRNA gene sequence analysis
Summary
A 4-Chloro-2-nitrophenol (4C2NP) decolourizing strain RKJ 700 was isolated from soil collected from a pesticide contaminated site of India and identified as Bacillus subtilis on the basis of the 16S rRNA gene sequence analysis. Resting cell studies showed that Bacillus subtilis RKJ 700 depleted 4C2NP completely with stoichiometric formation of 5C2MBZ. This is the first report of (i) the degradation of 4C2NP at high concentration (1.5 mM) and, (ii) the formation of 5C2MBZ by a soil bacterium. 4C2NP may undergo reduction with the formation of 4-chloro-2-aminophenol (4C2AP) [1] This reaction was catalyzed by either an oxygen sensitive reductase under anaerobic condition or an oxygen insensitive reductase under aerobic conditions [1]. The degradation of 4C2NP was catalyzed by an oxygenase with release of nitrite ion This reaction was observed under aerobic condition. The aims of this study are (i) to isolate a bacterium that can degrade high concentration of 4C2NP (1.0 mM or above) and (ii) study of metabolic pathway of 4C2NP
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