Decolourization and detoxification of monoazo dyes by laccase from the white-rot fungus Trametes versicolor

Abstract

The decolourization and detoxification of azo dyes (Orange 2, Acid Orange 6) by fungal laccase from Trametes versicolor were evaluated. For laccase catalysed reaction, the azonaphthol Orange 2, with 72.8% decolourization, was degraded more rapidly than the azobenzene Acid Orange 6, with 45.3%. The presence of hydroxyl group at o-position to azo bond in the structure of Orange 2 was more preferable than the presence of two hydroxyl groups at o- and p-positions to azo bond in Acid Orange 6. Although the laccase treatment was more effective for the Orange 2 decolourization, the toxicity of both monoazo dye solutions became less toxic for the prokaryote growth. The phytotoxicity of Orange 2 and Acid Orange 6 solutions after laccase treatment was decreased in the range of 41.2–64.3 %. Also, the photoxicity, as measured by the production of chlorophylls a and b by Chlorella vulgaris and Microcystis aeruginosa, was decreased by laccase treatment of selected monoazo dyes. Our results show that different dyes can be decolorized and detoxified by laccase from T. versicolor in a single step.