Decolourisation of Turquoise HGN by anaerobic bacterial isolates from rumen enrichments

Abstract

Anaerobic bacteria capable of decolourising Turquoise HGN dye were isolated by roll tube technique from enrichments inoculated with rumen fluid and were tested for their decolourisation efficiency under different conditions. The presence of glucose in the culture medium favoured the decolourisation and specific dye reduction activity (equivalent to the reduction of 1 mg dye. hr−1.mg−1 of protein), but both were inhibited in the presence of yeast extract. The maximum growth and dye reduction activity was recorded at pH 7.0 and 30°C, and were in the range of 36.4–97.4 U and 68–82 U, respectively with glucose as co-metabolite.