Abstract

A solid-state system to obtain rapid in vivo industrial dye decolourisation by the white-rot fungus Pleurotus pulmonarius is proposed. When cultivated on glucose\\ammonium tartrate–corncob solid-state medium, P. pulmonarius decolourised structurally different synthetic dyes (including azo, triphenylmethane, heterocyclic and polymeric dyes). Amido black, congo red, trypan blue, methyl green, remazol brilliant blue R (RBB), methyl violet, ethyl violet and brilliant cresyl blue were completely decolourised after 6 days of cultivation, while methylene blue and Poly R-478 were partially decolourised. The capability of fungal culture to decolourise industrial dyes appears to be due to high titres of laccase (480 U/ml) produced in response to the presence of soluble phenolics.

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