Decolorization of remazol brilliant blue R with laccase from Lentinus crinitus grown in agro-industrial by-products.

Patrícia H Almeida,Juliana S Do Valle,Giani A Linde,Juliana C Friedrich,Ana Carolina C De Oliveira,Genyfer P.N De Souza,Nelson B Colauto

doi:10.1590/0001-3765201820170458

Patrícia H Almeida, Juliana S Do Valle + Show 5 more

Open Access

https://doi.org/10.1590/0001-3765201820170458

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Abstract

Lentinus crinitus is a white-rot fungus that produces laccase, an enzyme used for dye decolorization. Enzyme production depends on cultivation conditions, mainly agro-industrial by-products. We aimed to produce laccase from Lentinus crinitus with agro-industrial by-products for dye decolorization. Culture medium had coffee husk (CH) or citric pulp pellet (CP) and different nitrogen sources (urea, yeast extract, ammonium sulfate and sodium nitrate) at concentrations of 0, 0.7, 1.4, 2.8, 5.6 and 11.2 g/L. Enzymatic extract was used in the decolorization of remazol brilliant blue R. CH medium promoted greater laccase production than CP in all evaluated conditions. Urea provided the greatest laccase production for CH (37280 U/L) as well as for CP (34107 U/L). In CH medium, laccase activity was suppressed when carbon-to-nitrogen ratio changed from 4.5 to 1.56, but the other nitrogen concentrations did not affect laccase activity. For CP medium, reduction in carbon-to-nitrogen ratio from 6 to 1.76 increased laccase activity in 17%. The peak of laccase activity in CH medium occurred on the 11th day (41246 U/L) and in CP medium on the 12th day (32660 U/L). The maximum decolorization within 24 h was observed with CP enzymatic extract (74%) and with CH extract (76%).

Highlights

There is a worldwide trend to develop more efficient productive chains, where the management of industrial residues favors the economic and environmental sustainability of processes
Laccase production occurred in Erlenmeyer flasks (250 mL) containing 100 mL of culture medium consisting of 1.5 g/L KH2PO4, 0.5 g/L MgSO4, 0.5 g/L KCl, 0.036 g/L FeSO4, 0.035 g/L ZnSO4 and agro-industrial by-products (50 g/L) such as coffee husk (CH) or citric pulp pellet (CP) as the only carbon source
Our study showed that L. crinitus did not produce manganese peroxidase (MnP) in the submerged cultivation using media added with two different agro industrial byproducts

Summary

Introduction

There is a worldwide trend to develop more efficient productive chains, where the management of industrial residues favors the economic and environmental sustainability of processes. The efficient utilization of agro-industrial by-products and wastes allows the appreciation of this material and represents an alternative to generate highlyaggregated value products with the recovery of. In Brazil, by-products from coffee and citric industries are abundant sources of nutrients and were chosen for this study. Brazil produced more than 51 million coffee bags in 2016 (CONAB 2017). Coffee husks have been utilized successfully in different bioprocesses to produce mushroom, citric acid, ethanol, aroma compounds, enzymes, to name but a few (Murthy and Naidu 2012). Citric pulp consists of a mixture of orange peels, seeds and bagasse from the orange juice processing. The yearly volume of this byproduct is approximately 2 million tons, and in 2016 the Brazilian industry produced more than 860 thousand tons of concentrated orange juice. Citric pulp is mainly utilized in animal feeding, production of citric acid (Torrado et al 2011), and pectin (Kaya et al 2014), besides being an excellent substrate for enzymatic production (Spier et al 2008)

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