Abstract
The aim of the present study is to analyze the ability of the white rot fungi Coriolus Versicolor (MTCC 138) in the decolorization of Cibacron Yellow S-3R, a recalcitrant azo reactive textile dye. The influencing parameters that affect the percentage of decolorization rates are optimized in batch mode. The optimal values of the parameters such as mycelia age, temperature, pH, initial dye concentration and carbon source concentration are found to be 7 days, 30°C, 5.4, 100 mg/l and 2 mg/l respectively. The maximum percentage of decolorization at the optimized conditions is found to be 90%. It is also conferred that there is substrate inhibition to fungal decolorization when initial dye concentration is greater than 100 mg/l.
Highlights
Though the number of studies on the biodegradation of dyestuffs have been steadily increasing in recent years, very few researches are reported for biodegradation of azo dyes, such as Cibacron Yellow S-3R using white rot fungi (Yesilada et al, 1998) and in this present research an attempt has been made to investigate the biological decolorization of the azo dye Cibacron Yellow S-3R using Coriolus Versicolor (MTCC 138)
Further increasing days of culture growth doesn’t make any appreciable change in the percentage of decolorization and 7 days of culture growth is found to be optimum for further studies
The same trend is observed by Duygu et al, (2005) for bio decolorization of Brilliant Blue using Funalia trogii
Summary
5 ml of the suspension is transferred to 250 ml flask containing 100 ml Sabouraud’s Dextrose Broth. The fungus is cultured at 30°C on slant Sabouraud’s Dextrose Agar and after a week a conidial suspension is prepared and utilized for the cultivation of the inoculums. It is incubated at 30°C for 5 days in a shaking incubator at 130 rpm. The contents of the flasks are sterilized for 20 min (1.5 atm, 121°C) and incubated in shaking incubator at 130 rpm.
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