Decolorization and biodegradation of reactive sulfonated azo dyes by a newly isolated Brevibacterium sp. strain VN-15

Elisangela Franciscon,Jonas Augusto Rizzato Paschoal,Lucia Regina Durrant,Felix Guillermo Reyes Reyes,Matthew James Grossman

doi:10.1186/2193-1801-1-37

Elisangela Franciscon, Jonas Augusto Rizzato Paschoal + Show 3 more

Open Access

https://doi.org/10.1186/2193-1801-1-37

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Journal: SpringerPlus	Publication Date: Oct 23, 2012
Citations: 151	License type: cc-by

Affiliation: Universidade Estadual de Campinas

Abstract

Azo dyes constitute the largest and most versatile class of synthetic dyes used in the textile, pharmaceutical, food and cosmetics industries and represent major components in wastewater from these industrial dying processes. Biological decolorization of azo dyes occurs efficiently under low oxygen to anaerobic conditions. However, this process results in the formation of toxic and carcinogenic amines that are resistant to further detoxification under low oxygen conditions. Moreover, the ability to detoxify these amines under aerobic conditions is not a wide spread metabolic activity. In this study we describe the use of Brevibacterium sp. strain VN-15, isolated from an activated sludge process of a textile company, for the sequential decolorization and detoxification of the azo dyes Reactive Yellow 107 (RY107), Reactive Black 5 (RB5), Reactive Red 198 (RR198) and Direct Blue 71 (DB71). Tyrosinase activity was observed during the biotreatment process suggesting the role of this enzyme in the decolorization and degradation process, but no-activity was observed for laccase and peroxidase. Toxicity, measured using Daphnia magna, was completely eliminated.

Highlights

Azo dyes account for about one-half of all dyes produced and are the most commonly used synthetic dyes in the textile, food, paper making, color paper printing, leather and cosmetic industries (Chang and Lin 2001)
The VN-15 strain was phylogenetically positioned in the genus Brevibacterium
The nucleotide alignment of the partial 16S rRNA gene sequence of this strain had identity values of 98 to 99%, with different Brevibacterium strains including the sequences of the strains B. linens, B. permense, B. epidermidis and B

Summary

Introduction

Azo dyes account for about one-half of all dyes produced and are the most commonly used synthetic dyes in the textile, food, paper making, color paper printing, leather and cosmetic industries (Chang and Lin 2001). The textile industry accounts for two-thirds of the total dyestuff market and during the dyeing process approximately 10% of the dyes used are released into the wastewater (Easton 1995). Textile dye wastewaters have high TOC, high salt content and extremes in pH, with reactive dye baths having high pH and acid dye baths have low pH (Golob et al 2005)

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