Abstract
Repair of UVC-induced DNA damage in Caenorhabditis elegans is similar kinetically and genetically to repair in humans, and it slows significantly in aging C. elegans.
Highlights
Caenorhabditis elegans is an important model for the study of DNA damage and repair related processes such as aging, neurodegeneration, and carcinogenesis
Evidence is building that many of these genes are homologous in function as well as sequence; mutations or RNA interference (RNAi) knockdown of apparent DNA repair homologs have produced genotoxin-sensitive phenotypes [7,9,10,11,12,13,14], and RNAi screens for genes that protect against mutations have identified DNA repair gene homologs in C. elegans [15]
Exposure to ultraviolet type C (UVC) radiation causes similar, dosedependent damage in the nuclear and mitochondrial genomes We adapted a quantitative polymerase chain reaction (QPCR) assay for analyzing gene-specific DNA damage and repair to C. elegans
Summary
Caenorhabditis elegans is an important model for the study of DNA damage and repair related processes such as aging, neurodegeneration, and carcinogenesis. We adapted a quantitative polymerase chain reaction assay to characterize repair of DNA damage induced by ultraviolet type C (UVC) radiation in C. elegans, and tested whether DNA repair rates were affected by age in adults. Http://genomebiology.com/2007/8/5/R70 powerful system that is increasingly used to study many human conditions that are affected by DNA damage and repair, including carcinogenesis [1,2], neurodegenerative diseases [3,4], and aging [5,6]. Homologs of many human DNA genes are present in the C. elegans genome [7,8], suggesting that this simple multicellular eukaryote might be a good model for the study of DNA repair processes in higher eukaryotes. The molecular pathways that mediate cellular response to DNA damage, including apoptosis, are fairly well conserved between C. elegans and humans [16,17]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
