Decision letter: Local mechanical forces promote polarized junctional assembly and axis elongation in Drosophila

Abstract

Tissues and organs form certain shapes that allow them to perform particular roles in the body. For example, the lungs form sacs that accommodate large volumes of air, while the skin forms a sheet to cover and protect our internal organs. One way to shape a tissue is for cells to swap places with their neighbours. During this rearrangement, the contacts between neighbouring cells break down before new contacts are formed with other cells. While the physical and molecular signals that guide the break down of cell contacts are well understood, less is known about how new contacts form. Early in development, animal embryos establish a head-to-tail 'axis' that helps to guide where each tissue and organ will form in the body. In fruit fly embryos, the cell rearrangements that drive this process involve cells exchanging places with their neighbours by gathering around a single point. These temporary cell clusters are then organised via new cell contacts that form parallel to the head-to-tail axis. Here, Yu and Fernandez-Gonzalez investigate the role of mechanical forces in forming new cell contacts as the head-tail axis elongates. The experiments show that disrupting the ability of the cells to generate mechanical forces inhibited the formation of new cell contacts and prevented cells from successfully swapping places. Conversely, when mechanical tension is applied at the rearrangement site, the assembly of new cell contacts happens faster. Furthermore, if the tension is applied in different orientations, new cell contacts form parallel to the direction of the mechanical force. Yu and Fernandez-Gonzalez thus show that local mechanical forces direct the assembly of new cell contacts as the head-to-tail axis forms. These forces are most likely generated by cell contractions that appear to create mechanical tension at sites of cell rearrangement. How such physical forces are converted into molecular signals remains a question for future work.