Deciphering the role of calcium peroxide on the fate of antibiotic resistance genes and mobile genetic elements during bioelectrochemically-assisted anaerobic composting of excess dewatered sludge

Abstract

The integration of bioelectrochemical system (BES) into conventional anaerobic composting process can accelerate the degradation of excess dewatered sludge (ES). However, no reports have been made concerning antibiotic resistance genes (ARGs) profile and abundance in BESs fuelled with ES, and the effect of CaO2 on the fate of ARGs within BESs treating excess sludge has rarely been investigated. The aim of this study was to investigate the effect of CaO2 and the bioelectrochemical process on the abundances of ARGs and MGEs and to unravel the mechanism of ARGs attenuation. Results showed that CaO2 addition could enhance the reduction of ARG levels in ES within AnCBE. The mean ARG abundances in the AnCBE samples with high CaO2 doses (0.4 g CaO2/g VSS and 0.5 g CaO2/g VSS) were significantly lower than the values in samples with other CaO2 doses. The abundances of lincosamide nucleotidyltransferase, macB, macrolide transporter ATP-binding proteins, and macrolide-efflux proteins significantly decreased with the increase in the CaO2 dose. Both CaO2 addition and bioelectrochemical assistance played important roles in shaping the ARG composition during the AnCBE process. The variation in the microbial community composition is the most important contributor to the variation in the ARGs composition. The abundances of Actinobacteria and Firmicutes explained 52.8% of the total ARG variance. Of the MGEs, the abundances of plasmids, insertion sequences, and integrons were all reduced in the sludge metagenomes. There was a significantly positive correlation between the abundances of sulI, sulII, tetG, and blaTEM and those of metal resistance genes (MRGs), which decreased significantly after the AnCBE process. This study revealed the potential of the combination of CaO2 and bioelectrogenesis for ARG attenuation.