Deciphering the photoactive species-directed antibacterial mechanism of bismuth oxychloride with modulated nanoscale thickness

Abstract

As an environmentally benign disinfection strategy, photocatalytic bacterial inactivation using nanoparticles involves photogenerated reactive species that cause cellular oxidative stress. Rationalising the structural performance of photocatalysts for the practical uses such as wastewater treatment has attracted significant attention; however, the contribution of reactive species to their photocatalytic antibacterial activities at the molecular and transcriptomic levels remains unclear. In this study, nontoxic bismuth oxychloride (BiOCl) photocatalysts with different nanoscale thicknesses, including nanosheets (Ns, ∼5.4 nm), nanoplates (Np, ∼1.8 nm), and ultra-nanosheets (Uns, ∼1.1 nm), were synthesised under hydrothermal conditions. Among the three samples, BiOCl Uns exhibited the most effective photocatalytic degradation efficiency with the calculated apparent rate constant of 0.0294 min−1, ∼4 times faster than that of Ns, whereas BiOCl Ns possessed the most pronounced bactericidal effect (5.4 log inactivation). Such findings indicate the distinct role of the photoactive species responsible for photocatalytic bacterial inactivation. Moreover, transcriptome analysis of Escherichia coli after photocatalytic treatment revealed that the underlying photocatalytic antibacterial mechanism at the genetic expression level involves cellular component biosynthesis, energy metabolism, and material transportation. Notably, the differences between BiOCl Ns and BiOCl Uns were significantly enriched in purine metabolism. Therefore, the cost-effective preparation of BiOCl nanosheets with nanoscale thickness-modulated photocatalytic antibacterial activity has remarkable potential for sustainable environmental and biomedical applications.