Deciphering the microbial patterns of anammox process under hexavalent chromium stress: Abundant and rare subcommunity respond differently

Abstract

This study aims to unravel the microbial responses to Cr(VI) stress in anaerobic ammonium oxidation (anammox) reactor. The result showed that anammox process could tolerate 2 mg/L Cr(VI) after acclimation, while 5 mg/L Cr(VI) stress resulted in significant inhibition on anammox bacterial activity. Ca. Jettenia was the predominant anammox genus, whose abundance showed a decreasing tendency with increasing Cr(VI) dosage. Cr(VI) addition resulted in significant and irreversible changes in microbial community structure, and increased the relative influence of stochastic processes on community assembly. Furthermore, rare subcommunity contributed greatly to biodiversity of whole community (90.35%), while abundant subcommunity were more similar to the whole community. Importantly, Cr(VI) exposure caused greater variations in rare subcommunity compared with abundant one, indicating that rare taxa were more sensitive to Cr(VI) stress. This was further confirmed by ABT model, which showed higher relative influence of Cr(VI) on rare subcommunity. In addition, results suggested that rare taxa play essential roles in whole community stability, because of their great contribution to species richness and community variations, and keystone roles in ecosystem network. Moreover, network analysis showed that conditionally rare taxa frequently and positively interacted with abundant taxa, which may contribute to the community resilience to Cr(VI) stress.