Deciphering the effect of novel bacterial exopolysaccharide-based nanoparticle cream against Propionibacterium acnes

Abraham P Karlapudi,Vijaya R Dirisala,Krishna P Kota,Vidya P Kodali,Sabiha S Shaik,N S Sampath Kumar

doi:10.1007/s13205-015-0359-5

Abraham P Karlapudi, Vijaya R Dirisala + Show 4 more

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https://doi.org/10.1007/s13205-015-0359-5

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Abstract

Acne vulgaris (acne) is a chronic inflammatory disease prevalent among adolescents and adults, with significant psychological effects. The aetiology of acne is multifactorial. Several pathophysiological associations have been identified in which Propionibacterium acnes plays a major role. This bacteria primarily affects areas containing oil glands including the face, back and trunk, where it causes the formation of seborrhoea and inflammatory lesions. The treatment methods currently in place have side effects. A novel alternative method with no side effects is hence required. In this study, we report the synthesis of an exopolysaccharide (EPS)-producing bacterial-based nanoparticle as a stable biocompatible material for drug delivery. We then evaluated the effectiveness of EPS-based nanoparticle cream against P. acnes. Our results demonstrate that EPS nanoparticles have great potential as a safe and effective topical treatment for acne vulgaris and other associated infections.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-015-0359-5) contains supplementary material, which is available to authorized users.

Highlights

Acne vulgaris is a chronic inflammatory disease of the sebaceous gland characterized by follicular hyperkeratinization and excessive colonization by Propionibacterium acnes (P. acnes) (Beylot et al 2014)
We report the synthesis of an exopolysaccharide (EPS)-producing bacterialbased nanoparticle as a stable biocompatible material for drug delivery
Our results demonstrate that EPS nanoparticles have great potential as a safe and effective topical treatment for acne vulgaris and other associated infections

Summary

Introduction

Acne vulgaris (acne) is a chronic inflammatory disease of the sebaceous gland characterized by follicular hyperkeratinization and excessive colonization by Propionibacterium acnes (P. acnes) (Beylot et al 2014). Acne affects the majority of adolescents, who develop inflammatory lesions such as papules, pustules and nodules, as well as other non-inflammatory lesions (Webster 2014). P. acnes activates immune reactions by releasing chemo-active agents that attract neutrophils, monocytes, lymphocytes, and stimulate the production of pro-inflammatory cytokines (Kistowska et al 2015). Over the past few decades, exopolysaccharides have gained attention for their bioactive properties such as stability, biodegradability and biocompatibility. Exopolysaccharides (EPS) are usually anionic heteropolysaccharides due to the presence of various constituents such as xanthan, alginate and glycans, etc. EPS produced by microorganisms vary in their composition and properties such as anti-bacterial, anticancer, anti-tumoral, anti-ulcer, anti-immune stimulation along with cholesterol-lowering ability (Kumar et al 2007; Raveendran et al 2013; Raposo et al 2014).

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