Abstract
Pollutants removal and genetic responses of simultaneous heterotrophic nitrification and aerobic denitrification (SHNAD) treating seawater-based wastewater were studied under ampicillin stress. Marine SHAND bacteria exhibited good tolerance to 10 mg/L ampicillin with nitrogen removal efficiency and organics removal efficiency of 94.5% and 82.6%, respectively. Besides, the half-inhibitory concentration of ampicillin on marine SHAND bacteria was 50 mg/L. The relative abundances of antibiotic resistance genes (ARGs) first decreased and then increased with ampicillin addition. The blaVIM played an important role to resist 25 mg/L ampicillin, which contributed to the recovery of pollutants removal. BlaSHV and blaTEM dominated ARG subtypes, which accounted for 96.6% of ARGs abundance. At 50 mg/L ampicillin, reactive oxygen species (ROS) production and cell numbers of apoptosis increased by 47.9% and 367.5%, respectively. The overproduction of ROS was stimulated by ampicillin, which caused bacterial cell apoptosis. Marine SHNAD bacteria produced more extracellular polymeric substances to resist ampicillin.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
