Abstract
Cyclodextrins (CDs) are yielded by cyclodextrin glycosyltransferase (CGTase) using starches or their derivatives as substrates. However, how starch fine structure affects it production is still ambiguous. This study aimed to decipher the relevance between the external chain length (ECL) and CD production. The waxy maize starch was hydrolyzed by β-amylase to prepare starchy substrates with regular gradient ECL for CGTase. The HPAEC results reflected that β-amylolysis lower the starch chain fractions with DP range of 13–24 by 30%, while the fractions with DP < 6 promoted by 30%. The molecular weight distribution and iodine binding results reflected that this process had limited impact on overall starch molecular size parameters. After CGTase conversion, it is interesting to find that the ECL was positively correlated with CD contents, which also has a high consistency among α-CD, β-CD, and γ-CD. This research would provide a favorable perspective for CD production improvement.
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