Abstract
The capacity of cellular components of Escherichia coli to metabolize DDT-14C was investigated. Washed membrane fractions were obtained by lysozyme treatment followed by osomotic shock. Gas chromatography with electron capture detection and thin-layer chromatography were used to measure the effect of exogenous Kreb’s cycle cofactors and intermediates on the metabolism of DDT by particulate components of the bacteria. Reductive dechlorination of DDT to TDE occurred in the membranous fraction of the bacterial cell and was stimulated by a factor (s) present in the cytoplasmic fraction. Isolated membranes produced substantial amounts of TDE only in the presence of exogenous flavin adenine dinucleotide (FAD), and the prior reduction of FAD is essential to the production of TDE. The presence of Kreb’s cycle energy inhibited TDE production, indicating that DDT does not accept electrons produced by the oxidation of Kreb’s cycle intermediates directly from the cytochrome system.
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