Decellularized Pig Kidney with a Micro-Nano Secondary Structure Contributes to Tumor Progression in 3D Tumor Model.

Tianqing Liu,Yi Nie,Zeren Jiao,Kedong Song,Bo Pan,Zilong Chen,Le Zheng,Yue Kang,Shuangjia Yang

doi:10.3390/ma15051935

Abstract

In spite of many anti-cancer drugs utilized in clinical treatment, cancer is still one of the diseases with the highest morbidity and mortality worldwide, owing to the complexity and heterogeneity of the tumor microenvironment. Compared with conventional 2D tumor models, 3D scaffolds could provide structures and a microenvironment which stimulate native tumor tissues more accurately. The extracellular matrix (ECM) is the main component of the cell in the microenvironment that is mainly composed of three-dimensional nanofibers, which can form nanoscale fiber networks, while the decellularized extracellular matrix (dECM) has been widely applied to engineered scaffolds. In this study, pig kidney was used as the source material to prepare dECM scaffolds. A chemical crosslinking method was used to improve the mechanical properties and other physical characteristics of the decellularized pig kidney-derived scaffold. Furthermore, a human breast cancer cell line (MCF-7) was used to further investigate the biocompatibility of the scaffold to fabricate a tumor model. The results showed that the existence of nanostructures in the scaffold plays an important role in cell adhesion, proliferation, and differentiation. Therefore, the pig kidney-derived matrix scaffold prepared by decellularization could provide more cell attachment sites, which is conducive to cell adhesion and proliferation, physiological activities, and tumor model construction.

Highlights

A certain pressure pressure was applied on the matrix, after the shape of the pigWhen kidney matrix was applied on the decellularized matrix, the shape of the pig kidney matrix did not did not change dramatically, indicating that the pig kidney matrix had good mechanicalchange indicating that was the pig matrix had subsequent good mechanical properties after properties afterdramatically, decellularization, which alsokidney reflected in the compression decellularization, which was reflected in the subsequent compression modulus detecmodulus detection results
To improve the mechanical properties of the decellularized matrix, the matrix matrix, the matrix was treated by ethyl carbonimide hydrochloride (EDC)/NHS, which could crosslink the amino group was treated by EDC/NHS, which could crosslink the amino group with the carboxyl group
The results showed that the porosity of the porcine kidney-derived matrix scaffolds decreased from 84.14 ± 3.20% to 81.09 ± 4.63% after crosslinking, which indicated a significant difference (p < 0.05) [38], As reported, decellularized extracellular matrix (dECM) derived from animal tissues exhibited a porous structure with a porosity around 80%, allowing higher cell adhesion and cell invasion [13,39]

Summary

Introduction

Lung cancer, and colorectal cancer are the three most prevalent cancers among women, which account for 50 percent of all new diagnoses. Breast cancer alone accounts for 30 percent of diagnosed cancer in women [1]. 1 in 8 (13%) women can be diagnosed with invasive breast cancer in their lifetime. By 2019, the number of new breast cancer cases in the United States had surpassed 250,000, and

Methods

Results

Conclusion