Abstract
This study was designed to test the ability of decellularized native matrices and primitive pluripotential stem cells to provide optimal conditions for native islets to secrete insulin in response to a glucose challenge. Adult rats were euthanized following the guidelines of MU‐IACUC. Adult rat pancreases were decellularized using detergents. Control cultures consisted of native matrices, naïve stem cells, and native islets, all cultured individually. Experimental groups consisted of native islets co‐cultured with naïve stem cells seeded on top of decellularized native matrices. Control cultures and experimental cultures were challenged with glucose. The culture media were assayed using a RIA specific for rat insulin. No detectable insulin was secreted by naïve stem cells or decellularized pancreatic matrices. The native pancreatic islets secreted ng insulin per ng DNA. Pancreatic islets co‐cultured with naïve stem cells and native matrices demonstrated increased insulin secretion in the range of mg insulin per ng DNA. The current study shows that co‐cultures of decellularized native matrices, naïve adult stem cells, and native islets exhibited greater than a 250‐fold increase in insulin secretion in response to a glucose challenge compared to native pancreatic islets alone. Funding was provided by LM & HO Young Estate Trust and Dragonfly Foundation.
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