Decellularization of kidney tissue: comparison of sodium lauryl ether sulfate and sodium dodecyl sulfate for allotransplantation in rat.

Mohammad Amin Keshvari,Amin Tamadon,Reza Shirazi,Iraj Nabipour,Mohammad Reza Miri,Arezoo Khoradmehr,Hossein Azari,Mahdi Muhaddesi,Sajad Daneshi,Pouya Behrouzi,Mandana Baghban,Alireza Afshar,Mehdi Mahmudpour

doi:10.1007/s00441-021-03517-5

Abstract

An automatic decellularization device was developed to perfuse and decellularize male rats' kidneys using both sodium lauryl ether sulfate (SLES) and sodium dodecyl sulfate (SDS) and to compare their efficacy in kidney decellularization and post-transplantation angiogenesis. Kidneys were perfused with either 1% SDS solution for 4h or 1% SLES solution for 6h. The decellularized scaffolds were stained with hematoxylin and eosin, periodic acid Schiff, Masson's trichrome, and Alcian blue to determine cell removal and glycogen, collagen, and glycosaminoglycan contents, respectively. Moreover, scanning electron microscopy was performed to evaluate the cell removal and preservation of microarchitecture of both SDS and SLES scaffolds. Additionally, DNA quantification assay was applied for all groups in order to measure residual DNA in the scaffolds and normal kidney. In order to demonstrate biocompatibility of the decellularized scaffolds, human umbilical cord mesenchymal stromal/stem cells (hUC-MSCs) were seeded on the scaffolds. In addition, the allotransplantation was performed in back muscle and angiogenesis was evaluated. Complete cell removal in both SLES and SDS groups was observed in scanning electron microscopy and DNA quantification assays. Moreover, the extracellular matrix (ECM) architecture of rat kidney in the SLES group was significantly preserved better than the SDS group. The hUC-MSCs were successfully migrated from the cell culture plate surface into the SDS and SLES decellularized scaffolds. The formation of blood vessels was observed in the kidney in both SLES and SDS decellularized kidneys. The better preservation of ECM than SDS introduces SLES as the solvent of choice for kidney decellularization.

Highlights

The kidney diseases have many diversity and complexity such as acute kidney injury (AKI), chronic kidney disease (CKD) and end-stage renal disease (ESRD) (Romagnani et al 2017)
We demonstrated that both sodium lauryl ether sulfate (SLES) and sodium dodecyl sulfate (SDS) could be promising tools in kidney tissue engineering
The hUC-MSCs were successfully migrated from the cell culture plate surface into the SDS and SLES decellularized scaffolds

Summary

Introduction

The kidney diseases have many diversity and complexity such as acute kidney injury (AKI), chronic kidney disease (CKD) and end-stage renal disease (ESRD) (Romagnani et al 2017). In order to treat ESRD patients, renal replacement therapy methods such hemodialysis, peritoneal dialysis and renal transplantation have been introduced (Davison and Moss 2016; Gander et al 2019). These treatments are expensive, time-consuming and not available to the majority of people. The rising number of patients requiring renal replacement therapy alongside the shortage of acceptable donors are concerning issues (Meersch and Zarbock 2018). Chronic kidney diseases and end stage renal disease are growing threats worldwide. An automatic decellularization device was developed to perfuse and decellularize male rats' kidneys using both sodium lauryl ether sulfate (SLES) and sodium dodecyl sulfate (SDS) and to compare their efficacy in kidney decellularization and post-transplantation angiogenesis

Methods

Results

Discussion

Conclusion