Abstract
Background and Aim:Kidney regeneration is required for dogs with end-stage renal failure. Decellularization is one of the bioengineering techniques, which involves the removal of all tissue cells and cellular components and conservation of the extracellular matrix (ECM). Studies in rats have shown that decellularized kidney has regenerative potential; however, there are no reports on renal decellularization in dogs. Here, we showed the decellularization of the canine kidney.Materials and Methods:The renal artery of the cadaveric canine kidney was cannulated and the whole kidney was frozen at −80°C. After completely thawing, it was perfused with physiological saline and sodium dodecyl sulfate (0.5%, 6 h) through the cannulated renal artery to achieve decellularization. To assess the efficiency of the decellularization protocol, histological and immunohistochemical analysis of decellularized kidney was performed.Results:The results of hematoxylin and eosin (H and E) staining revealed that the decellularized canine kidney had no apparent cellular components. In addition, 4’,6-diamidino-2-phenylindole (DAPI) staining showed no visible nuclear components within the whole decellularized kidney. Therefore, both H and E and DAPI staining showed decellularization of the canine kidney. Our decellularization protocol also preserved the basement membrane of glomerulus, shown by periodic acid methenamine silver, periodic acid–Schiff, fibronectin, and collagen type IV stain.Conclusion:Our decellularization protocol could eliminate cellular components and remaining native ECM structures of canine kidney. These results could promote further research into canine kidney regeneration, which may be the first small step to regenerate the canine kidney waiting for renal transplantation.
Highlights
Kidney disease is an important problem in dogs and cats
Our decellularization protocol could eliminate cellular components and remaining native extracellular matrix (ECM) structures of canine kidney. These results could promote further research into canine kidney regeneration, which may be the first small step to regenerate the canine kidney waiting for renal transplantation
To prove the efficiency of our decellularization protocol, the cadaveric canine kidney was evaluated by macrophotography during decellularization
Summary
Kidney disease is an important problem in dogs and cats. Apart from renal transplantation, there is no treatment to improve the renal function of patients with end-stage renal failure [6]. Some reports have indicated that old age and severe azotemia may increase mortality rates after renal transplantation [6,7]. Renal transplantation in the early stages is desired to improve prognosis. The fundamental shortage of donors has limited renal transplantation in veterinary medicine. Kidney regeneration is required for dogs with end-stage renal failure. Studies in rats have shown that decellularized kidney has regenerative potential; there are no reports on renal decellularization in dogs. We showed the decellularization of the canine kidney
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
