Abstract
DNA sequencing with a solid-state nanopore requires a reduction of the translocation speeds of single-stranded DNA (ssDNA) over 10 μs/base. In this study, we report that a nanometre-sized bead structure constructed around a nanopore can reduce the moving speed of ssDNA to 270 μs/base by adjusting the diameter of the bead and its surface chemical group. This decelerating effect originates from the strong interaction between ssDNA and the chemical group on the surface of the bead. This nanostructure was simply prepared by dip coating in which a substrate with a nanopore was immersed in a silica bead solution and then dried in an oven. As compared with conventional approaches, our novel method is less laborious, simpler to perform and more effective in reducing ssDNA translocation speed.
Highlights
Nanopore DNA sequencing is undergoing rapid development for its potential advantages compared with existing DNA sequencing methods[3]
In a previous study[12], among many methods aimed at decelerating DNA11,13–18 as it passes through a nanopore, we selected a method that narrows the nanopore because it is compatible with the above-mentioned sequencing measurement
Double-stranded DNA can interact with nanofibres as it translocates through the mesh, and it can be decelerated to approximately 1 μ s/base
Summary
Nanopore DNA sequencing is undergoing rapid development for its potential advantages (i.e., long reads, low cost and high speed1,2) compared with existing DNA sequencing methods[3]. This method effectively decelerates single-stranded DNA (ssDNA) translocation up to 1 μ s/base[9,12]. Densely packed beads provide the nanoporous structure, and the chemical functional group on the surface of the bead interacts with the DNA molecule.
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