Abstract
Ferritin iron can be reduced by O .− 2, released, and form a Fe(II)-chelator complex. However, the thermodynamic influence of the chelator may disturb the reaction balance. We therefore excluded the chelator and measured instead the effect of ferritin on the decay of O .− 2, monitored by direct spectrophotometry at pH 9.5. Ferritin, but not apoferritin, accelerated the decay of O − 2. Ferritin iron was apparently the responsible agent. The effect of ferritin was maintained after several bursts of O .− 2, and the ratio degraded O .− 2/released Fe(II) greatly exceeded one, consistent with a catalytic reaction.
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