Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates.

Nuttaporn Samart,Santosh Kumar,Heide A Murakami,Zeyad Arhouma,Dean C Crick,Debbie C Crans

doi:10.3389/fchem.2018.00519

Abstract

51V NMR spectroscopy is used to document, using speciation analysis, that one oxometalate is a more potent growth inhibitor of two Mycobacterial strains than other oxovanadates, thus demonstrating selectivity in its interaction with cells. Historically, oxometalates have had many applications in biological and medical studies, including study of the phase-problem in X-ray crystallography of the ribosome. The effect of different vanadate salts on the growth of Mycobacterium smegmatis (M. smeg) and Mycobacterium tuberculosis (M. tb) was investigated, and speciation was found to be critical for the observed growth inhibition. Specifically, the large orange-colored sodium decavanadate (V10) anion was found to be a stronger inhibitor of growth of two mycobacterial species than the colorless oxovanadate prepared from sodium metavanadate. The vanadium(V) speciation in the growth media and conversion among species under growth conditions was monitored using 51V NMR spectroscopy and speciation calculations. The findings presented in this work is particularly important in considering the many applications of polyoxometalates in biological and medical studies, such as the investigation of the phase-problem in X-ray crystallography for the ribosome. The findings presented in this work investigate the interactions of oxometalates with other biological systems.

Highlights

IntroductionPolyoxometalates (POMs) are a class of compounds that have been investigated in a range of biological and biomedical systems, as their effects in cell culture studies and in vivo suggest that these compounds have potential for use as therapeutic agents (Moskovitz and Group, 1988; Hill et al, 1990; Rhule et al, 1998; Aureliano and Crans, 2009; Fraqueza et al, 2012; Aureliano et al, 2013, 2016; Wang et al, 2013; Aureliano and Ohlin, 2014; Leon et al, 2014; Kioseoglou et al, 2015; Shah et al, 2015; Sun et al, 2016; Fu et al, 2018; Gumerova et al, 2018)
The formation of decavanadate has been demonstrated in yeast (S. cerevisiae) and makes this anion a desirable system to understand in greater detail (Willsky et al, 1984b, 1985)
Information on cellular uptake by vanadium species is of interest, and the studies presented here compare the effect of monomeric vanadate and decavanadate

Summary

Introduction

Polyoxometalates (POMs) are a class of compounds that have been investigated in a range of biological and biomedical systems, as their effects in cell culture studies and in vivo suggest that these compounds have potential for use as therapeutic agents (Moskovitz and Group, 1988; Hill et al, 1990; Rhule et al, 1998; Aureliano and Crans, 2009; Fraqueza et al, 2012; Aureliano et al, 2013, 2016; Wang et al, 2013; Aureliano and Ohlin, 2014; Leon et al, 2014; Kioseoglou et al, 2015; Shah et al, 2015; Sun et al, 2016; Fu et al, 2018; Gumerova et al, 2018). Speciation studies are important in this regard, and different species and possibilities must be considered when investigating the mode of action of systems that are not thermodynamically stable (Aureliano and Crans, 2009; Levina et al, 2017a). Even if the speciation is characterized, the active species and mode of action of these complex systems can be non-trivial to interpret (Willsky et al, 1984b, 1985, 2011; Delgado et al, 2005; Crans et al, 2011; Postal et al, 2016; Jakusch and Kiss, 2017). The formation of decavanadate has been demonstrated in yeast (S. cerevisiae) and makes this anion a desirable system to understand in greater detail (Willsky et al, 1984b, 1985)

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