Decaffeinated light-roasted green coffee and green tea extract combination improved metabolic parameters and modulated inflammatory genes in metabolic syndrome rats

Mohammad Saifur Rohman,Dwi Adi Nugroho,Nashi Widodo,Mifetika Lukitasari,Risa Ramadhiani,Inggita Kusumastuty,Nur Ida Panca Nugrahini

doi:10.12688/f1000research.27921.1

Mohammad Saifur Rohman, Dwi Adi Nugroho + Show 5 more

Open Access

https://doi.org/10.12688/f1000research.27921.1

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Abstract

Background: Individually, green tea and green coffee have been extensively studied for mitigation of metabolic syndrome (MS) in both rats and humans; however, their combined effect requires further investigation. Thus, we compared the metabolic effect of combining green tea and decaffeinated light roasted green coffee on MS in rats. Methods: An MS animal model was constructed by feeding Sprague-Dawley rats with a high-fat-high-sucrose (HFHS) diet for eight weeks and a low dose of streptozotocin (STZ) injection at week 2. Rats fed with HFHS diets and injected with STZ successfully developed MS phenotypes, indicated by higher body weight, systolic blood pressure, plasma triglyceride level, plasma fasting blood glucose level, and lower plasma HDL-C level, compared to those fed with a normal chow diet. Subsequently, MS rats were continuously fed with HFHS and divided into four groups: MS rats, MS with 300 mg/bw.t green tea extract (GT), MS with 200 mg/bw.t green coffee extract (GC), and MS with combined green tea and green coffee extract (CM) for nine weeks. Results: Combining green tea and green coffee have synergistic effects on reducing plasma fasting blood glucose and triglyceride level. Inflammatory markers both in plasma and liver tissue robustly decreased in CM group rats. However, the reduction of systolic blood pressure was observed only in GT and CM groups. Moreover, all treatment resulted in an increase in plasma HDL-C level in MS rats. Conclusions: Our data highlighted that, in MS animal models, combined green tea and decaffeinated light roasted green coffee augment their several individual beneficial effects of improved metabolic parameters and modulated inflammatory genes.

Highlights

Metabolic syndrome is a global public health concern
Animal characteristics Assessment of body weight, systolic blood pressure, fasting blood glucose level, triglyceride and HDL cholesterol plasma level of combination HFHS diet and a low dose of STZ injection revealed the presence of metabolic syndrome symptoms as classified by the NCEP-ATP III criteria
During the nine following weeks, the rats that met NCEP-ATP III criteria were subjected to HFHS alone or HFHS with 300 mg/BW of green tea extract (GT), 200 mg/BW of decaffeinated light roasted green coffee extract (GC), and combination of those extracts (CM) (Figure 1a)

Summary

Introduction

Metabolic syndrome is a global public health concern. The prevalence of this disease is estimated to be about one-quarter of the world population. Previous studies have shown that nuclear factor-kappa B (NF-kB) signalling is a crucial pathway in chronic low-grade inflammation process and has been studied extensively in the context of obesity and the metabolic syndrome[2,3]. Management of this disease consists of a dual approach that combines lifestyle modification and pharmacological intervention on those risk factors. Results: Combining green tea and green coffee have synergistic effects on reducing plasma fasting blood glucose and triglyceride level. Inflammatory markers both in plasma and liver tissue robustly

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Conclusion