Debaryomyces hansenii L2-enriched diet enhances the immunity status, gene expression and intestine functionality in gilthead seabream (Sparus aurata L.)

Abstract

This study evaluates the effects of the dietary administration of the live yeast Debaryomyces hansenii strain L2 on the immune responses of gilthead seabream for 4 weeks. Cellular immune parameters were measured from serum and head-kidney leucocytes respectively. The expression levels of immune-associated genes were quantified by real-time polymerase chain reaction. In addition, the profile of intestinal microbiota was studied by denaturing gradient gel electrophoresis. The results showed that seabream fed a diet containing D. hansenii had significantly increased cellular immune parameters. The yeast-supplemented diet up-regulated the expression of most seabream genes at week 2 and down-regulated all of them at week 4, except in the head-kidney. Finally, a reduction in the diversity of the intestinal microbiota was detected in those specimens receiving the yeast-supplemented diet. These results support the idea that the live yeast D. hansenii strain L2 stimulates the immune system of gilthead seabream.