Abstract
A technique is described in which the entire effluent from a microcolumn is led directly into the flow-stream of an autoanalyzer and mixed with a fluorogenic substrate in buffer. The result is a continuous trace of the elution profile of enzyme activity on the recorder chart. Good separations of four N-acetyl-β- d-hexosaminidase components of human tissues were achieved in less than 30 min on 5 μl samples from 1% w v homogenates.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have