Abstract
When Streptomyces violaceoruber grows together with Streptomyces sp. MG7‐G1, it reacts with strongly induced droplet production on its aerial mycelium. Initially the metabolite profile of droplets from S. violaceoruber in co‐culture with Streptomyces sp. MG7‐G1 was compared to samples from S. violaceoruber in single‐culture by using high‐performance liquid chromatography‐mass spectrometry (HPLC‐MS). Then, the exudate from agar plates of co‐cultures and single cultures (after freezing and thawing) was also analysed. Several compounds were only observed when S. violaceoruber was grown in co‐culture. Based on their high‐resolution ESI mass spectra and their comparable retention times to the calcium‐dependent antibiotics (CDAs) produced by S. violaceoruber, the new compounds were suspected to be deacylated calcium‐dependent antibiotics (daCDAs), lacking the 2,3‐epoxyhexanoyl residue of CDAs. This was verified by detailed analysis of the MS/MS spectra of the daCDAs in comparison to the CDAs. The major CDA compounds present in calcium ion‐supplemented agar medium of co‐cultures were daCDAs, thus suggesting that Streptomyces sp. MG7‐G1 expresses a deacylase that degrades CDAs.
Highlights
MG7-G1 provokes S. violaceoruber to form more than 15 times more droplets than when grown alone, we investigated whether the interaction of both strains resulted in an alteration of their metabolite profiles and led to the
MG7-G1 and S. violaceoruber, we compared the metabolite profiles of S. violaceoruber grown in co-culture with Streptomyces sp
Streptomyces coelicolor A3(2)[13a] and produced by the closely related S. violaceoruber DSM 40783,[14] several new compounds 5, 6, 7 and 8 were found in samples from the cocultures that eluted at a similar retention time as the calcium-dependent antibiotics (CDAs) (Scheme 1, Figure 1)
Summary
MG7-G1 results in the deacylation of calcium-dependent antibiotics (CDAs)[13] from S. violaceoruber. Streptomyces coelicolor A3(2)[13a] and produced by the closely related S. violaceoruber DSM 40783,[14] several new compounds 5, 6, 7 and 8 were found in samples from the cocultures that eluted at a similar retention time as the CDAs (Scheme 1, Figure 1). Fragmentation pattern of daCDAs in comparison to the CDAs using high-resolution electrospray tandem fragmentation mass spectrometry of the [M + H] + quasimolecular ions.
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