Abstract

Bioethanol is regarded as one of the most promising biofuel from renewable sources. The development of ethanol production from lignocellulosic material is thus considered as the second-generation biofuel technology. Lignocelluloses account for the majority of the total biomass present in the world. To initiate the production of industrially important products from cellulosic biomass, bioconversion of the cellulosic components into fermentable sugars is necessary. A variety of microorganisms including bacteria and fungi may have the ability to degrade the cellulosic biomass to glucose monomers. The main aim of this work is the production of bioethanol from Deoiled Rice Bran by using Pichia kudriavzevii RCEF4907 and to optimize various parameters affecting the production of bioethanol. The production of bioethanol from Deoiled Rice Bran can be estimated by specific gravity method. In this study, various physical and biological methods carried out for the production of bioethanol and analysed optimum parameters such as incubation period, temperature, pH, nutrient supplements and biological pretreatment. Thus under the optimum conditions of temperature 25 °C, pH 6.5, and on 3rd day of fermentation, P. kudriavzevii RCEF4907 produced maximum bioethanol 10.5% while in biological pretreatment 10.8% bioethanol produced and in case of nutrient analysis, 1ml of 1% Urea solution maximum production of bioethanol 11.4% was obtained.

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