Abstract
Polyethylene glycol-mediated cell fusion between the hypoxanthine phosphoribosyl transferase (HPRT) deficient XO mouse embryonal carcinoma cell line, PSA-6TG1, and thymus or spleen lymphocytes from the normal male WKA/Hok rat gave rise to 35 somatic hybrid cultures. Hybrid cells being products of either a 1:1 or a 2:1 fusion invariably had morphological characteristics of endodermal cells from early embryos. BrdU-acridine orange (AO) fluorescence microscopy revealed de novo appearance of a late or early replicating, presumably genetically inactivated, mouse X chromosome in a substantial proportion of virtually tetraploid (XXY) or hexaploid (XXXY) hybrid cells.
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