Abstract
Parsley is an important biennial Apiaceae species that is widely cultivated as herb, spice, and vegetable. Previous studies on parsley principally focused on its physiological and biochemical properties, including phenolic compound and volatile oil contents. However, little is known about the molecular and genetic properties of parsley. In this study, 23,686,707 high-quality reads were obtained and assembled into 81,852 transcripts and 50,161 unigenes for the first time. Functional annotation showed that 30,516 unigenes had sequence similarity to known genes. In addition, 3,244 putative simple sequence repeats were detected in curly parsley. Finally, 1,569 of the identified unigenes belonged to 58 transcription factor families. Various abiotic stresses have a strong detrimental effect on the yield and quality of parsley. AP2/ERF transcription factors have important functions in plant development, hormonal regulation, and abiotic response. A total of 88 putative AP2/ERF factors were identified from the transcriptome sequence of parsley. Seven AP2/ERF transcription factors were selected in this study to analyze the expression profiles of parsley under different abiotic stresses. Our data provide a potentially valuable resource that can be used for intensive parsley research.
Highlights
Parsley (Petroselinum crispum L.) is a biennial Apiaceae species that is native to the Mediterranean coast and widely cultivated in Europe and Japan
The curly leaf and plain leaf types are cultivated for their foliage, whereas root parsley is grown as a root vegetable [1]
RNA-Seq is a feasible and economical modern sequencing technology for obtaining transcriptomic data in a short time. This technology can detect new transcripts that correspond to existing genomic sequences; it can be used to generate sequence resources for gene discovery, expression, and annotation, and for discovering simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) in non-model organisms without a reference genome [32,33,34]
Summary
Parsley (Petroselinum crispum L.) is a biennial Apiaceae species that is native to the Mediterranean coast and widely cultivated in Europe and Japan. Parsley is widely utilized in the cosmetic, medicinal, and food industries because it is an excellent source of phenolic compounds, volatile oils, vitamins, and nutrients [2,3,4]. Global challenges, such as climate change, environmental degradation, and toxic waste, subject plants to various stresses during growth. Transcription factors function in gene expression by combining DNA-binding and cis-acting elements [8,9]. Many transcription factors, such as AP2/ERF, NAC, bZIP, and WRKY, are related to stress resistance in plants [10,11,12,13]
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