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Abstract
Southern corn rust is a destructive maize disease caused by Puccinia polysora Underw that can lead to severe yield losses. However, genomic information and microsatellite markers are currently unavailable for this disease. In this study, we generated a total of 27,295,216 high-quality cDNA sequence reads using Illumina sequencing technology. These reads were assembled into 17,496 unigenes with an average length of 1015 bp. The functional annotation indicated that 8113 (46.37%), 1933 (11.04%) and 5516 (31.52%) unigenes showed significant similarity to known proteins in the NCBI Nr, Nt and Swiss-Prot databases, respectively. In addition, 2921 (16.70%) unigenes were assigned to KEGG database categories; 4218 (24.11%), to KOG database categories; and 6,603 (37.74%), to GO database categories. Furthermore, we identified 8,798 potential SSRs among 6653 unigenes. A total of 9 polymorphic SSR markers were developed to evaluate the genetic diversity and population structure of 96 isolates collected from Guangdong Province in China. Clonal reproduction of P. polysora in Guangdong was dominant. The YJ (Yangjiang) population had the highest genotypic diversity and the greatest number of the multilocus genotypes, followed by the HY (Heyuan), HZ (Huizhou) and XY (Xinyi) populations. These results provide valuable information for the molecular genetic analysis of P. polysora and related species.
Highlights
Southern corn rust is a destructive maize disease caused by Puccinia polysora Underw that can lead to severe yield losses
The transcriptome sequences provide a valuable genomic resource for the molecular study of P. polysora, and the findings provide the first perspective into the P. polysora population structure in Guangdong Province
The remaining high-quality reads were assembled into 10,539 transcripts with an average length of 1,229 bp and an N50 of 2,083 bp (Table 1)
Summary
Southern corn rust is a destructive maize disease caused by Puccinia polysora Underw that can lead to severe yield losses. The YJ (Yangjiang) population had the highest genotypic diversity and the greatest number of the multilocus genotypes, followed by the HY (Heyuan), HZ (Huizhou) and XY (Xinyi) populations. These results provide valuable information for the molecular genetic analysis of P. polysora and related species. The disease has caused several serious yield losses in various countries, including West Africa (50%6), the Philippines (80–84%21), Brazil (40%22), the United States (30–50%3) and China (42–53%23). The function of teliospores in the life cycle remains mystery, they could be of significance in assuring extended survival of the pathogen. Due to the lack of evidence, C ammack provisionally classified P. polysora as a microcyclic and autoecious hemiform
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