Abstract
The spinyhead croaker (Collichthys lucidus) is a commercially important fish species, which is mainly distributed in the coastal regions of China. However, little is known about the molecular regulatory mechanism underlying reproduction in C. lucidus. A de novo transcriptome assembly in brain, liver, ovary and testis tissues of C. lucidus was performed. Illumina sequencing generated 60,322,004, 57,044,284, 60,867,978 and 57,087,688 clean reads from brain, liver, ovary and testis tissues of C. lucidus, respectively. Totally, 131,168 unigenes with an average length of 644 bp and an N50 value of 1033 bp were assembled. In addition, 1288 genes were differentially expressed between ovary and testis, including 442 up-regulated and 846 down-regulated in ovary. Functional analysis revealed that the differentially expressed genes between ovary and testis were mainly involved in the function of sexual reproduction, sex differentiation, development of primary male sexual characteristics, female gamete generation, and male sex differentiation. A number of genes which might be involved in the regulation of reproduction and sex determination were found, including HYAL and SYCP3 and BMP15. Furthermore, 35,476 simple sequence repeats (SSRs) were identified in this transcriptome dataset, which would contribute to further genetic and mechanism researches. De novo transcriptome sequencing analysis of four organs of C. lucidus provides rich resources for understanding the mechanism of reproductive development of C. lucidus and further investigation of the molecular regulation of sex determination and reproduction of C. lucidus.
Highlights
Collichthys lucidus, which belongs to Perciformes, is an economically important fish with small somatotype and usually lives in the benthic zone of coastal waters [1]
The similarity distribution of the top hits showed that 61% of the mapped sequences had similarities more than 80%, while 37% of the hits had similarities ranging from 40% to 80% (Fig 1A)
To investigate pathways connected to reproduction and gonad development, we focused on differentially expressed unigenes between ovary and testis
Summary
Collichthys lucidus, which belongs to Perciformes, is an economically important fish with small somatotype and usually lives in the benthic zone of coastal waters [1]. C. lucidus is appreciated by customers because of its good quality of meat, which contributing to fishery. Genes involved in sex determination and reproduction in Collichthys lucidus production [3]. Statistics showed that it reached 222.6t per year [4]. Studies revealed that the resources of C. lucidus have been declining due to the overfishing and the damage of environment [5]. It is necessary to carry out genetic research and exploit reproductive techniques to increase fish production for resource protection and ecological balance
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