Abstract
Spodoptera is an important polyphagous agricultural insect pest in the tropical world. The genomic details are limited to understand the pest biology at molecular level. In the present study, we sequenced and assembled the transcriptome from Sf21 cells into a non redundant set of 24,038 contigs of ~ 47.38 Mb in size. A total of 26,390 unigenes were identified from the assembled transcripts and their annotation revealed the prevalent protein domains in Sf21 cells. The present study would provide a resource for gene discovery and development of functional molecular markers to understand the biology of S. frugiperda.ReviewersThis article was reviewed by Dr. Thiago Motta Venancio and Prof. Michael Gray.Electronic supplementary materialThe online version of this article (doi:10.1186/s13062-015-0072-7) contains supplementary material, which is available to authorized users.
Highlights
Spodoptera is an important polyphagous agricultural insect pest in the tropical world
Considering the agricultural and economical importance of S. frugiperda, our group generated the draft assembly of genomic DNA from Sf21 cells, a cell line derived from the ovary of S. fruigperda [1]
To characterize the transcriptome of Sf21 cells, total RNA isolated from Sf21 monolayer was used to prepare the library and subjected to high throughput sequencing on Illumina HiSeq 2000 platform
Summary
Spodoptera is an important polyphagous agricultural insect pest in the tropical world. The transcripts encode SSRs of higher in length (>6 nt) and the complete list of SSRs with their frequency and respective sequences are provided in Additional file 5.
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