Abstract
Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) is a major pest affecting Solanaceae plants in Asian countries. In this study, we sequenced the ovary and testis transcriptomes of H. vigintioctopunctata to identify gonad-related genes. Comparison of the unigene sequences in ovary and testis libraries identified 1,421 and 5,315 ovary- and testis-specific genes, respectively. Among the ovary-specific genes, we selected the RC2-like and PSHS-like genes to investigate the effects of gene silencing on the mortality, percentage infertility, pre-oviposition period, fecundity, daily number of eggs laid, and hatching rate in female adults. Although the percentage mortality and infertility of females did not differ significantly among dsRNA treatments, fecundity was significantly reduced in the dsRC2-like and dsPSHS-like treatment groups. Moreover, the pre-oviposition period was markedly prolonged in response to dsPSHS-like treatment. This is the first reported RNA sequencing of H. vigintioctopunctata. The transcriptome sequences and gene expression profiles of the ovary and testis libraries will provide useful information for the identification of gonad-related genes in H. vigintioctopunctata and facilitate further research on the reproductive biology of this species. Moreover, the gonad-specific genes identified may represent candidate target genes for inhibiting the population growth of H. vigintioctopunctata.
Highlights
Henosepilachna vigintioctopunctata (Fabricius) (Coleoptera: Coccinellidae) is a widely distributed beetle in Asian countries, including China, India, Japan, and Korea [1,2,3,4]
Hvi.gviingtiinotcitoocptuopnuctnacttaatoav, awrey saenqduetnecsetids lciDbrNarAiesl.ibFroalrlioews ifnrogmquoavliatryiecsonatnrdol,te2s6t9e,s27u6s,i9n6g2 abnp Iolflucmleianna rHeaiSdesqwTMere2r5e0t0riesveeqdueanfcter. reImnotvoitnagl, l2o7w0,9q4u8a,0li8ty8 brepaodfsranwdrteraimdsmwinergeagdeanpetreartse.dDferotamilssioxfHth. evisgeinqtuioecntcoipnugncatnadtasouvbasreyqaunedntteasstsiesmliblryaraieres. sFhoollwown ingTqaublaeli1ty. control, 269,276,962 bp of clean reads were retrieved after removing low-quality readsDaenndotvroimasmseinmgbaldyaopftethrse. cDleatanilrseoafdtshegesneqeruaetnecdin6g9,a8n5d4 suunbisgeeqnueesnwt aitshseamnbNly5a0roefsh12o8w4nbipn aTnabdlean1. average length of 780 bp (Table 1)
SPSS v. 21.0 (IBM Corp., Armonk, NY, USA) was used for all statistical analyses. This is the first study in which the RNA sequencing of H. vigintioctopunctata has been conducted
Summary
Henosepilachna vigintioctopunctata (Fabricius) (Coleoptera: Coccinellidae) is a widely distributed beetle in Asian countries, including China, India, Japan, and Korea [1,2,3,4] This species is considered to be a phytophagous pest that mainly causes damage to Solanaceae plants, eggplants, along with other crops such as potatoes, pumpkins, and rock melons [5]. Both the adult and larval stages of H. vigintioctopunctata feed on the leaves of these plants by scraping the leaf cuticle, thereby promoting the development of irregular transparent spots or perforations in the injured leaves, which later become brown depression marks. Given the current requirement for negative growth in pesticide use in China, as stipulated in the 2019 “No 1 Central Document,” there is an urgent need to develop effective new pest control methods that target H. vigintioctopunctata based on novel modes of action
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