Abstract
Bletilla striata polysaccharide (BSP) is the main component of Bletilla striata, which has important pharmacological and pharmacological effects; however, due to the lack of genetic data, the metabolic pathways of BSP remain unclear. For this study, 11 representative resources of B. striata were analyzed, and the BSP contents of the different samples were significantly different; however, the monosaccharide composition of BSP was glucose and mannose. The representative samples were selected to observe their life history in situ, which were then divided and cultured in a greenhouse. Finally, samples from various organs of different plants were combined for transcriptome sequencing using the Illumina system. Our results summarized the BSP metabolic pathway, and we found that there were eight enzyme genes involved in biosynthesis, but these genes showed tissue specificity. Following qRT-PCR validation and comparative analysis, manA showed the highest expression; however, there were significant differences between the two germplasm resources in which the BSP content was significantly different, while UGP2, GPI, PMM, and GMPP had significant differences between the two samples. In summary, this study lays the foundation for further research into BSP metabolism and other physiological processes at the molecular level.
Highlights
Bletilla striata, typically known as an important species of the Bletilla genus, is a significant medicinal compound for the treatment of many diseases, as recorded by Chinese pharmacopoeia (Chen et al, 2018; Zhang et al, 2019a)
Transcriptome sequencing was performed by mixing the different organs of different plants
The results indicated that the polysaccharides of B. striata were derived from glucose and mannose; the metabolic processes of UDP-glucose (UDP-Glc) and GDP-mannose (GDP-Man) were the key metabolic pathway of Bletilla striata polysaccharide (BSP)
Summary
Typically known as an important species of the Bletilla genus, is a significant medicinal compound for the treatment of many diseases, as recorded by Chinese pharmacopoeia (Chen et al, 2018; Zhang et al, 2019a). It is an ornamental flower but belongs to the Orchidaceae family (He et al, 2017). Polysaccharides are one of the main components, which plays a variety of biological functions (Wang and Meng, 2015; Zhang et al, 2019b). It is necessary to conduct in-depth genetic research to discover its genes and their functionalitiess
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