Abstract
To enrich the molecular data of Pinus bungeana Zucc. ex Endl. and study the regulating factors of different morphology controled by apical dominance. In this study, de novo assembly of transcriptome annotation was performed for two varieties of Pinus bungeana Zucc. ex Endl. that are obviously different in morphology. More than 147 million reads were produced, which were assembled into 88,092 unigenes. Based on a similarity search, 11,692 unigenes showed significant similarity to proteins from Picea sitchensis (Bong.) Carr. From this collection of unigenes, a large number of molecular markers were identified, including 2829 simple sequence repeats (SSRs). A total of 158 unigenes expressed differently between two varieties, including 98 up-regulated and 60 down-regulated unigenes. Furthermore, among the differently expressed genes (DEGs), five genes which may impact the plant morphology were further validated by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The five genes related to cytokinin oxidase/dehydrogenase (CKX), two-component response regulator ARR-A family (ARR-A), plant hormone signal transduction (AHP), and MADS-box transcription factors have a close relationship with apical dominance. This new dataset will be a useful resource for future genetic and genomic studies in Pinus bungeana Zucc. ex Endl.
Highlights
Pinus bungeana Zucc. ex Endl. is an endemic species to China, with high economic and ecological values for landscaping and afforestation
Approximately 148 million paired end reads were generated by high throughput sequencing
37.21 Gb bases were obtained with 91.56% Q30 bases with a GC content of 45.85% (Table 1)
Summary
Pinus bungeana Zucc. ex Endl. is an endemic species to China, with high economic and ecological values for landscaping and afforestation. Is an endemic species to China, with high economic and ecological values for landscaping and afforestation. Owing to long-term over-exploitation, natural forest of. Has been severely damaged and natural populations are mainly found on the top of hills with poor conditions usually isolated and fragmented. The plant morphology, which is the most visible difference, has been commonly used for genetic diversity studies. Its regulation and secondary metabolites should be focused on. Sizable numbers of molecular markers and genes data from the genome are playing an increasingly important role in population genomic studies of fine-scale genetic variation and the genetic basis of traits [1]. Very limited genomic data is available on Pinus bungeana Zucc. Very limited genomic data is available on Pinus bungeana Zucc. ex Endl
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