Abstract
The Venus clam, Cyclina sinensis, is one of the most important bivalves in China. In recent years, increasing expansive morbidity has occurred in breeding areas, imposing significant losses on the national economy. To understand the molecular mechanisms of immune-related genes, we analyzed and sequenced hemolymph samples that were injected with two pathogenic microorganisms using the Illumina Miseq system. After trimming, more than 12 M PE reads with an average length greater than 410 bp were assembled into 70,079 transcripts with a mean length of 980 bp. Using a homology analysis, 102 (135 transcripts) potentially immune-related genes were identified, and most of them exhibited a similar pattern in both samples. These data indicated that the response of the clam to both types of bacterial infection might follow a similar molecular mechanism. Using the TreeFam method, 9,904 gene families and 1,031 unique families of the clam were preliminarily classified in comparison to five related species. A significant number of SSRs were identified, which could facilitate the identification of polymorphisms in Venus clam populations. These datasets will improve our knowledge of the molecular mechanisms driving the immune response to bacterial infection in clam populations and will provide basic data about clam breeding and disease control.
Highlights
The Venus clam, Cyclina sinensis, is one of the most important bivalves in Chinese marine aquaculture
After the data were trimmed, a total of 6,694,694 high-quality reads (2,808,369,022 base pairs) from sample M stimulated by Vibrio anguillarum and 5,483,161 high-quality reads (2,314,488,797 base pairs) from sample T stimulated by Micrococcus luteus were obtained
The results revealed that, compared with other species, C. gigas shared the most gene families (6,643/67.07%) with C. sinensis
Summary
The Venus clam, Cyclina sinensis, is one of the most important bivalves in Chinese marine aquaculture This clam possesses many advantages as a popular seafood, including rapid growth, salt and temperature resistance, pollution tolerance, and a high survival ratio after prolonged removal from the water[1,2]. This clam species is distributed across most of China’s coastline and has a huge consumer market in China, South Korea and Japan. An important cause of increased morbidity is invasion by pathogens[3,4]. Vibrio disease is the most significant cause of death in many marine organisms; the pathogenic taxa mainly include Vibrio anguillarum, Vibrio vulnificus, Vibrio alginolyticus and Vibrio harveyi
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