Abstract
Direct regeneration from mature embryo axes was achieved without intervening of callus phase in four chickpea varieties on the Media MS and B5 supplemented with combination of BAP, NAA and Kinetin. Hundred percent regeneration capacity was exhibited by commercially grown Vijay and Vishal varieties. There was considerable variation in umber of multiple shoot production by different varieties. Profuse rooting was obtained on the medium containing 0.5 and 1.0 mg/l IBA. This protocol is optimized for complete plant regeneration of chickpea for genetic transformation.
Highlights
Chickpea (Cicer arietinum L.) is one among the highly cultivated and consumed legume throughout the world due to its high protein contents
Present paper describes the complete regeneration in chickpea varieties
In the present report describe an approach for inducing a high frequency de novo shoot regeneration of four genotypes of C. arietinum
Summary
Chickpea (Cicer arietinum L.) is one among the highly cultivated and consumed legume throughout the world due to its high protein contents. Many wild species possess the wealth of agronomically desirable genes which are sexually incompatible to cultivated varieties and difficult to transfer. Earlier attempts are made to develop some resistance using conventional breeding methods involving wild relatives of chickpea, but without much success [1]. Despite in vitro regeneration from shoot meristem [2,3], immature cotyledons [4] and through embryo genesis from immature cotyledons [5], leaflet callus [6,7,8], influence of genotype regeneration capacity poorly studied. Present paper describes the complete regeneration in chickpea varieties
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